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Composite

Part:BBa_I712050:Design

Designed by: Sasa Jereb   Group: iGEM07_Ljubljana   (2007-10-23)


CMV promoter - RBS - T7 RNA polymerase - NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3316
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Genomic DNA was isolated form BL21 and PCR was performed to attach RBS and NLS.


Source

Genomic DNA of BL21 (E.coli strain)

References